Properties of monolithic silica columns for HPLC.

نویسندگان

  • Hiroshi Kobayashi
  • Tohru Ikegami
  • Hiroshi Kimura
  • Takeshi Hara
  • Daisuke Tokuda
  • Nobuo Tanaka
چکیده

Monolithic silica columns and their use in high peak-capacity HPLC separations are reviewed. Monolithic silica columns can potentially provide higher overall performance than particle-packed columns based on the variable external porosity and variable through-pore size/skeleton size ratios. The high permeability of monolithic silica columns resulting from the high porosity is shown to be advantageous to generate large numbers of theoretical plates with long capillary columns. High permeability together with the high stability of the network structures of silica allows their use in high-speed separations required for a second-dimension column in two dimensional HPLC. Disadvantages of monolithic silica columns are also described.

برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

ثبت نام

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

منابع مشابه

Application of Monolithic Silica Column for HPLC Separation of Estrogens

A monolithic silica column has recently been widely used for HPLC separation analysis. Compared with the particle−packed column (conventional column), the monolithic silica column eluted the analyte with a smaller retention factor (k), lower pressure and satisfactory separation [1]. However, the monolithic silica column has not been widely used for the HPLC or LC/MS analysis of steroids. These ...

متن کامل

Monolithic LC columns.

Recently, monolithic columns consisting of one piece of an organic polymer or silica with flow-through pores have been reported in the literature. These materials provide better stability and, more important, higher performance than conventional columns packed with particles. Although they can be prepared by various methods, monolithic columns prepared by the direct polymerization of monomers c...

متن کامل

Comparing monolithic and fused core HPLC columns for fast chromatographic analysis of fat-soluble vitamins.

HPLC stationary phases of monolithic and fused core type can be used to achieve fast chromatographic separation as an alternative to UPLC. In this study, monolithic and fused core stationary phases are compared for fast separation of four fat-soluble vitamins. Three new methods on the first and second generation monolithic silica RP-18e columns and a fused core pentafluoro-phenyl propyl column ...

متن کامل

Monolithic silica-based capillary reversed-phase liquid chromatography/electrospray mass spectrometry for plant metabolomics.

Application of C18 monolithic silica capillary columns in HPLC coupled to ion trap mass spectrometry detection was studied for probing the metabolome of the model plant Arabidopsis thaliana. It could be shown that the use of a long capillary column is an easy and effective approach to reduce ionization suppression by enhanced chromatographic resolution. Several hundred peaks could be detected u...

متن کامل

Study of RP HPLC Retention Behaviours in Analysis of Carotenoids

For determination of selected carotenoids, various types of columns for high-performance liquid chromatography (HPLC) with different properties have been used. The characteristics of the laboratory-used packing material containing monomeric alkyl-bonded phases (C18, C30) and phenyl as well as phenyl-hexyl stationary phases were studied. The retention data of the examined compounds were used to ...

متن کامل

ذخیره در منابع من


  با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید

برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

ثبت نام

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

عنوان ژورنال:
  • Analytical sciences : the international journal of the Japan Society for Analytical Chemistry

دوره 22 4  شماره 

صفحات  -

تاریخ انتشار 2006